Educational Materials

10 Questions to Ask Your Apheresis Provider

Groundbreaking Cellular and Immunotherapy Discoveries
Begin Here 

From research to clinical trials, the success of your drug development project relies heavily on quality starting material. Having performed over 250,000 apheresis collections, HemaCare understands the unique needs of our customers while also adhering to cell product manufacturing best practices. With this expertise, we developed the 10 Questions to Ask Your Apheresis Provider guide to help you ask the right questions and be confident that your prospective partner’s goals are aligned with your own.

 

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Understand the Differences Between Myeloid and Lymphoid Cell Types

cell-lineage_landing-page

Receive a FREE poster developed by HemaCare showing the cell lineage of lymphoid and myeloid stem cells!

Download Free Lymphoid Poster

Download Free Myeloid Poster

Poster Size:
22″ x 17″

 

Are You Using Human Primary Cells?

 

Human primary cells are directly derived from the tissue, they are not transformed and non immortalized. Primary cells closely mimic characteristics similar to in vivo conditions – they are nearest to the “real thing”. HemaCare provides human primary cells derived from normal and mobilized peripheral blood, bone marrow, and cord blood and can isolate these cells using either positive selection or negative selection.

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Positive vs. Negative Selection

Any cell type can be isolated with either positive or negative selection techniques. In all cell selection strategies, a small magnetic bead is coupled to a specific antibody and that antibody binds to the target cell or cells.

Positive selection is the fastest way to magnetically label and isolate cells. With positive selection, the target cells are magnetically labeled and during isolation, the unlabeled cells are washed away and the magnetically labelled cells are collected. Typically, cells isolated via positive selection offers greater purity and recovery due to the specificity of the reaction.

With negative selection, all cells except the target cells are magnetically labelled leaving the target cells untouched. Negative selection is most often used when multiple cells types are desired, when there are no specific antibodies available for the target cells, or when binding of the antibody to the target cell is not desired. Negative selection normally results in lower purity since it is impossible to design a perfect cocktail to target all cells that do not carry the target cell molecule; though the difference in purity is minimal.

 

HLA Typing

Convention of HLA naming

Convention for HLA allele naming. The figure illustrates the meaning of each component of an HLA allele name. Courtesy of Prof Steven Marsh, Anthony Nolan; Research Institute, London, United Kingdom (www.hla.alleles.org). Eduardo Nunes et al. Blood 2011; 118:e180-e183

HLA Type refers to the unique set of proteins called human leukocyte antigens(HLA), also known as major histocompatibility complex (MHC).  These proteins, which contain peptide-binding clefts at the amino-terminal ends,  are present on each individual cell and direct the immune system to recognize self from foreign.  This allows the body to protect itself by recognizing and attacking something that does not belong to it, such as bacteria or viruses.

Applications:

  • research in T cell immunology,
  • flow cytometric work on HLA multimers, and
  • most functional cellular assays (i.e. ELISPOT)
  • vaccine and drug development and in clinical trials
  • identifying treatment for infectious diseases, autoimmune diseases, and cancer

Available Test Options

  • Low Resolution = A DNA-based typing result at the level of the digits composing the first field in the DNA-based nomenclature
  • High Resolution = a set of alleles that encode the same protein sequence for the region of the HLA molecule called the antigen binding site and that exclude alleles that are not expressed as cell-surface proteins. The antigen binding site includes domain 1 and domain 2 of the class I α polypeptides, and domain 1 of the class II α and domain 1 of the class II β polypeptide chains.

HLA-typed Donors and Cell Types

Approximately 90% of HemaCare’s diverse, active donor base has low resolution typing data. In addition, we strive to ensure that our inventory of cryopreserved cells are highly characterized with HLA type, blood type, CMV status, among other donor demographics that allows you to select the best cell lot for your needs!
Save Time. Save Money.

Download our HLA Information Sheet here for more information.