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10 Questions to Ask Your Apheresis Provider

From research to clinical trials, the success of your drug development project relies heavily on quality starting material. Having performed over 250,000 apheresis collections, HemaCare understands the unique needs of our customers while also adhering to cell product manufacturing best practices. With this expertise, we developed the 10 Questions to Ask Your Apheresis Provider guide to help you ask the right questions and be confident that your prospective partner’s goals are aligned with your own.


Application Note

Cryopreserved Leukopaks Maintain Cell Viability and Functionality: A Solution for Cell Therapy Logistics

Fresh leukopaks maintain good viability and functionality for the first 24 hours of transport. However, beyond 48 hours, the number of viable cells decreases dramatically. When considering global transportation and the cell therapy supply chain, the need for a practical shipping option is crucial. This application note evaluates HemaCare's cryopreserved leukopaks frozen on day 0 compared to fresh leukopaks at 24 hours.


Case Study

T-Cell Therapy Development Studies Rely on HemaCare Leukopaks to Facilitate Donor Sourcing

A case study featuring a European-based cell therapy company where the Lead Technical Scientist discusses how HemaCare met the challenge of obtaining high-quality leukopaks that met specific donor criteria while ensuring functionality of immune cells is not lost during shipment to the United Kingdom.


Case Study

Cell Therapy Company Rexgenero Utilizes HemaCare’s Bone Marrow Products for Development of their Critical Limb Ischemia Therapeutic

A case study featuring Rexgenero where Dr. de Jong discusses her experience in working with HemaCare products. The company is developing a number of cell-based therapeutics for the treatment of diseases such as peripheral artery disease and critical limb ischemia. During process development, healthy donor material is used as a stand-in for patient material and therefore it’s critical that variability be kept to a minimum.


White Paper

Regulatory T Cell Modulation of Immune Suppression

Regulatory T cells (Treg) are immune cells that help maintain balance in the immune system to prevent the destruction of healthy tissue, leading to inflammation and autoimmune disease. Understanding how Treg cells themselves are suppressed or activated is a major question being studied in current research and the primary goal of Treg focused cell therapy research. This white paper will discuss Tregs role in current research and clinical applications, isolation and cryopreservation best practices, and other factors that can significantly influence potency and downstream impact of cellular therapies.


Case Study

Precision Immunotherapy Program at Argos Therapeutics Utilizes HemaCare Leukopak Products for their Therapeutic Cell Manufacturing Workflow

A case study featuring Argos Therapeutics where Dr. Miesowicz discusses the challenges in finding raw materials for personalized immunotherapy. Donors are critical to the development process and reliable sourcing can be crucial to positive downstream results. Dr. Miesowicz emphasized that one of the main reasons he chooses HemaCare time and time again is because HemaCare supplies high quality products and goes above and beyond when coordinating development efforts.


Infographic

Primary Cells vs. Cell Lines

Human primary cells are directly derived from the tissue, they are not transformed and non immortalized. Primary cells closely mimic characteristics similar to in vivo conditions – they are nearest to the “real thing”. This infographic is a helpful visual breakdown of the difference between primary cells and cell lines.


Brochure

Research Use Biologics

General overview brochure highlighting HemaCare's research use only products and services.


Brochure

Cell Therapy Services

General overview brochure highlighting HemaCare's GMP-compliant products and services to support global cell therapy from product and process development through commercialization.


Poster

Myeloid Cell Lineage

Easy-to-follow overview of cell types derived from myeloid cells and highlights of the culture conditions to differentiate each cell.


Poster

Lymphoid Cell Lineage

Easy-to-follow overview of cell types derived from lymphoid cells and highlights of the culture conditions to differentiate each cell.


Protocol

How to Thaw Cryopreserved Cells

Thawing cryopreserved cells properly is crucial to ensure the viability and functionality of the cells. Using good technique and working quickly ensures that a high proportion of the cells survive the procedure.


Protocol

How to Activate Human Neutrophils

Neutrophils are a very sensitive cell type and can be easily activated by reagents. Activation of neutrophils is not an all or nothing phenomenon and each function has its own threshold for a response. This protocol provides step-by-step instructions on how to activate neutrophils.


Protocol

How to Cryopreserve Human Primary Cells

Cells are a valuable resource and replacement is expensive and time consuming. Therefore, freezing and preserving extra fresh cells or cells that are not needed immediately for processing for long-term storage is vitally important.


Protocol

How to Isolate Peripheral Blood Mononuclear Cells (PBMCs) Using a Density Gradient Reagent

The preparation of a leukopak prior to performing isolation of PBMCs is crucial to ensure good starting material for your project. This protocol provides step-by-step instructions on how to purify PBMCs from a leukopak.


Protocol

How to Thaw Cells in a Cryopreserved Leukopak

Thawing cryopreserved cells properly is crucial to the viability and functionality of the cells. Using good technique and working quickly ensures that a high proportion of the cells survive the procedure.


Protocol

How to Remove HypoThermosol® from Fresh Cells

HypoThermosol® is a novel, engineered, optimized hypothermic storage and shipping media product designed to provide maximum storage and shipping stability for biologics at 2-8°C. This protocol discusses how to remove HypoThermosol® if cells were shipped with this media.