Pan monocytes (which include classical, intermediate, and non-classical monocytes) are responsible for phagocytosis of foreign substances in the body and are capable of killing infected host cells via antibody-mediated cellular cytotoxicity. Under the right culture conditions, monocytes can differentiate into dendritic cells, Langerhans cells, or macrophages.
Pan monocytes are isolated from mononuclear cells by negative selection using immunomagnetic cell separation procedures.
Use fresh pan monocytes immediately upon receipt. Be sure to remove HypoThermosol® prior to use. If not used immediately, temporarily store cells at 0-4°C or freeze for long-term storage.
For cryopreserved pan monocytes, either prepare cells for long-term storage in Liquid Nitrogen vapor phase or thaw for use. Storage in liquid phase nitrogen is NOT recommended. Short-term storage of cells (less than 2 weeks) at -80°C is acceptable but should be minimized to ensure maximum stability. Once thawed, samples must be used immediately.
Testing: Donors are tested for HBV, HCV, HIV, HTLV, WNV, Trypanosoma cruzi, and Syphilis.