Neutrophils are the most abundant type of white blood cells produced by the bone marrow and form an essential part of the innate immune system. Neutrophils are one of the first-responders of inflammatory cells arriving to the site of injury within minutes of trauma. The mature neutrophil lives only a few hours or perhaps a little longer after migrating to the tissues. A large number of neutrophils are held in reserve in the bone marrow to accommodate for their short lifespan.
Neutrophils are derived from a Dextran red cell sedimented population of cells. Following red cell sedimentation, granulocytes are separated using a density gradient and red cell lysis. Cells are then purified through the use of indirect selection.
For cryopreserved neutrophils, either prepare cells for long-term storage in Liquid Nitrogen vapor phase or thaw for use. Storage in liquid phase nitrogen is NOT recommended. Short-term storage of cells (less than 2 weeks) at -80°C is acceptable but should be minimized to ensure maximum stability. Once thawed, samples must be used immediately.
Testing: Donors are tested for HBV, HCV, HIV, HTLV, WNV, Trypanasoma cruzi, and Syphilis.