Neutrophils

$530.00$1,260.00

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10 x 106 PB011C-1
$530.00 Cryopreserved
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25 x 106 PB011C-2
$1,260.00 Cryopreserved
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Description

Product Overview:

Neutrophils are the most abundant type of white blood cells produced by the bone marrow and form an essential part of the innate immune system. Neutrophils are one of the first-responders of inflammatory cells arriving to the site of injury within minutes of trauma. The mature neutrophil lives only a few hours or perhaps a little longer after migrating to the tissues. A large number of neutrophils are held in reserve in the bone marrow to accommodate for their short lifespan.

Neutrophils are derived from a Dextran red cell sedimented population of cells. Following red cell sedimentation, granulocytes are separated using a density gradient and red cell lysis. Cells are then purified through the use of indirect selection.

Storage Conditions:

For cryopreserved neutrophils, either prepare cells for long-term storage in Liquid Nitrogen vapor phase or thaw for use. Storage in liquid phase nitrogen is NOT recommended. Short-term storage of cells (less than 2 weeks) at -80°C is acceptable but should be minimized to ensure maximum stability. Once thawed, samples must be used immediately.

Testing: Donors are tested for HBV, HCV, HIV, HTLV, WNV, Trypanasoma cruzi, and Syphilis.

Neutrophils Flow Data

CD45 versus PI flow data
CD45 and Propidium Iodide (PI) are used to assess purity and viability of PBMCs. CD45 is expressed on all leukocytes and is used to indicate purity of the white blood cell (WBC) component. Because PI cannot pass through the membrane of live cells to stain DNA, it serves as an indicator of viability. Representative data shown here indicates purity of 95.09% – meaning >95% of cells in this sample are WBCs. (May not be indicative of received samples.)

CD15 versus CD11b flow data
CD15 is a surface antigen found on all neutrophils. CD11b is surface protein involved in adhesion between a number of cells and is expressed on the surface of monocytes, macrophages, and granulocytes. When comparing levels of CD11b versus CD15, the purity of the sample for neutrophils can be assessed. This graph shows a representative data set with purity of 99.32%. (Actual purity may vary somewhat from sample to sample.)

Certificate of Analysis

Cryopreserved Peripheral Blood Neutrophils

Protocols

How to Activate Human Neutrophils
How to Thaw Cryopreserved Cells