Naïve CD4 Helper T Cells

$895.00

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5 x 106 PB0445RAC-1
$895.00 Cryopreserved
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Description

Product Overview:

Naïve CD4 T cells have differentiated from bone marrow, and have successfully undergone the central tolerance processes of positive and negative selection in the thymus. They are considered mature but inactivated, responding to novel pathogens in the immune system and initiate the immune response. They are used to elicit brand new immune responses in in vitro and in vivo studies.

Naïve CD4+ T cells are isolated from leukopaks by positive selection using immunomagnetic cell separation procedures. Negative selection is also available at your request.

Storage Conditions:

For cryopreserved naïve CD4 T cells, either prepare cells for long-term storage in Liquid Nitrogen vapor phase or thaw for use. Storage in liquid phase nitrogen is NOT recommended. Short-term storage of cells (less than 2 weeks) at -80°C is acceptable but should be minimized to ensure maximum stability. Once thawed, samples must be used immediately.

Testing: Donors are tested for HBV, HCV, HIV, HTLV, WNV, Trypanasoma cruzi, and Syphilis.

Naïve CD4 Helper T Cells Flow Data

CD45 versus PI flow data
CD45 and Propidium Iodide (PI) are used to assess purity and viability of PBMCs. CD45 is expressed on all leukocytes and is used to indicate purity of the white blood cell (WBC) component. Because PI cannot pass through the membrane of live cells to stain DNA, it serves as an indicator of viability. Representative data shown here indicates purity of 95.09% – meaning >95% of cells in this sample are WBCs. (May not be indicative of received samples.)

CD45 versus CD3 flow data
Assessing CD3 expression versus CD45 in the isolated cell sample elucidates the purity of the sample for CD3+ T cells. This graph shows a representative data set with purity of 96.75% after isolation. (Actual purity may vary somewhat from sample to sample.)

CD3 versus CD4 flow data
CD4 is a co-receptor found specifically on helper T cells. Assessing CD4 versus CD3, a T cell specific marker, helps to assess both purity and specificity of the isolation procedure for the CD4 population. This data shows a representative data set with purity of 95.78%. (Actual purity may vary somewhat from sample to sample.)

CD45 versus CD45RA flow data
Naïve/non-activated T cells express CD45RA, the long isoform of CD45 leukocyte common antigen, on their surface until activation. CD45RA is used as a marker of naïve cells and when compared to total CD45 expression, shows the purity of a sample for naïve T cells. This graph shows a representative data set with purity of 99.75%. (Actual purity may vary somewhat from sample to sample.)

CD4 versus CD45RA flow data
Naïve/non-activated T cells express CD45RA, the long isoform of CD45 leukocyte common antigen, on their surface until activation. CD45RA is used as a marker of naïve cells and when compared to total CD4 expression, shows the purity and specificity of a sample for naïve CD4+ T cells. This graph shows a representative data set with purity of 93.15%. (Actual purity may vary somewhat from sample to sample.)

Certificate of Analysis

Cryopreserved Peripheral Blood Naive CD4 T Cells

Protocols

How to Thaw Cryopreserved Cells