Naïve CD4 T cells have differentiated from bone marrow, and have successfully undergone the central tolerance processes of positive and negative selection in the thymus. They are considered mature but inactivated, responding to novel pathogens in the immune system and initiate the immune response. They are used to elicit brand new immune responses in in vitro and in vivo studies.
Naïve CD4+ T cells are isolated from mononuclear cells by positive selection using immunomagnetic cell separation procedures. Negative selection is also available at your request.
For cryopreserved naïve CD4 T cells, either prepare cells for long-term storage in Liquid Nitrogen vapor phase or thaw for use. Storage in liquid phase nitrogen is NOT recommended. Short-term storage of cells (less than 2 weeks) at -80°C is acceptable but should be minimized to ensure maximum stability. Once thawed, samples must be used immediately.
Testing: Donors are tested for HBV, HCV, HIV, HTLV, WNV, Trypanasoma cruzi, and Syphilis.