Naïve CD3 T Cells

$1,990.00

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10 x106 PB0345RAC-1
$1,990.00 Cryopreserved
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Description

Product Overview:

A naïve T cell is a mature T cell that has differentiated in the bone marrow. Unlike memory T cells, naïve T cells have not acquired an activated phenotype, therefore naïve T cells can respond to unfamiliar pathogens that have not been previously encountered and initiate an immune response. As a result, these naïve T cells can acquire the phenotype and ultimately differentiate into a memory T cell.

Naïve T cells are isolated by depleting CD45RO+ cells via immunomagnetic bead separation from a freshly collected leukopheresis product. Naïve T cells are phenotypically classified as CD45+, CD3+, and CD45RA+.

Storage Conditions:

For cryopreserved naïve CD3 T cells, either prepare cells for long-term storage in Liquid Nitrogen vapor phase or thaw for use. Storage in liquid phase nitrogen is NOT recommended. Short-term storage of cells (less than 2 weeks) at -80°C is acceptable but should be minimized to ensure maximum stability. Once thawed, samples must be used immediately.

Testing: Donors are tested for HBV, HCV, HIV, HTLV, WNV, Trypanasoma cruzi, and Syphilis.

Naïve CD3 T Cells Flow Data

CD45 versus PI flow data
CD45 and Propidium Iodide (PI) are used to assess purity and viability of PBMCs. CD45 is expressed on all leukocytes and is used to indicate purity of the white blood cell (WBC) component. Because PI cannot pass through the membrane of live cells to stain DNA, it serves as an indicator of viability. Representative data shown here indicates purity of 95.09% – meaning >95% of cells in this sample are WBCs. (May not be indicative of received samples.)

CD45 versus CD3 flow data
Assessing CD3 expression versus CD45 in the isolated cell sample elucidates the purity of the sample for CD3+ T cells. This graph shows a representative data set with purity of 96.75% after isolation. (Actual purity may vary somewhat from sample to sample.)

CD8 versus CD4 flow data
CD4 and CD8 are co-receptors that can be used to distinguish the two major classes of T cells – helper and cytotoxic, respectively. The ratio of CD4 and CD8 cells can be used as a marker of immune activation and immune senescence. Representative data indicates a typical CD4/CD8 ratio that is around 2 but may vary depending on the health of donor. (May not be indicative of received samples.)

CD45 versus CD45RA flow data
Naïve/non-activated T cells express CD45RA, the long isoform of CD45 leukocyte common antigen, on their surface until activation. CD45RA is used as a marker of naïve cells and when compared to total CD45 expression, shows the purity of a sample for naïve T cells. This graph shows a representative data set with purity of 99.75%. (Actual purity may vary somewhat from sample to sample.)

CD3 versus CD45RA flow data
Naïve/non-activated T cells express CD45RA, the long isoform of CD45 leukocyte common antigen, on their surface until activation. CD45RA is used as a marker of naïve cells and when compared to total CD3 expression, a T cell specific surface marker, shows the purity and specificity of the T cell population within the sample. This graph shows a representative data set with purity of 96.28%. (Actual purity may vary somewhat from sample to sample.)

Certificate of Analysis

Cryopreserved Peripheral Blood Naïve CD3 T Cells

Protocols

How to Thaw Cryopreserved Cells