A naïve T cell is a mature T cell that has differentiated in the bone marrow. Unlike memory T cells, naïve T cells have not acquired an activated phenotype, therefore naïve T cells can respond to unfamiliar pathogens that have not been previously encountered and initiate an immune response. As a result, these naïve T cells can acquire the phenotype and ultimately differentiate into a memory T cell.
Naïve T cells are isolated by depleting CD45RO+ cells via immunomagnetic bead separation from a freshly collected leukopheresis product. Naïve T cells are phenotypically classified as CD45+, CD3+, and CD45RA+.
For cryopreserved naïve CD3 T cells, either prepare cells for long-term storage in Liquid Nitrogen vapor phase or thaw for use. Storage in liquid phase nitrogen is NOT recommended. Short-term storage of cells (less than 2 weeks) at -80°C is acceptable but should be minimized to ensure maximum stability. Once thawed, samples must be used immediately.
Testing: Donors are tested for HBV, HCV, HIV, HTLV, WNV, Trypanasoma cruzi, and Syphilis.