Mobilized peripheral blood mononuclear cells (PBMCs) are isolated from leukopaks collected in HemaCare’s FDA-registered collection centers following cGMP and cGTP collection guidelines from IRB consented healthy human donors. Donors are injected with G-CSF (Neupogen® – FDA approved), which stimulates the bone marrow to produce a large number of hematopoietic and progenitor stem cells and mobilizes them into the peripheral blood stream. This mononuclear cell-rich leukapheresis product is extracted using continuous flow centrifugal technology directly into the collection bag. Mobilized mononuclear cells are purified by a density gradient centrifugation and are available in fresh or cryopreserved format.
Fresh mobilized peripheral blood mononuclear cells (PBMCs) must be used immediately upon receipt. Be sure to remove HypoThermosol® prior to use. If not used immediately, temporarily store cells at 0-4°C or freeze for long-term storage.
For cryopreserved mobilized peripheral blood mononuclear cells, either prepare cells for long-term storage in Liquid Nitrogen vapor phase or thaw for use. Storage in liquid phase nitrogen is NOT recommended. Short term storage of cells (less than 2 weeks) at -80°C is acceptable but should be minimized to ensure maximum stability. Once thawed, samples must be used immediately.
Testing: Donors are tested for HBV, HCV, HIV, HTLV, WNV, Trypanasoma cruzi, and Syphilis.