Mobilized Peripheral Blood CD34+ Stem/Progenitor Cells; G-CSF

$0.00$6,800.00

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Contains:Autologous plasma, HypoThermosol®, or customer specific media upon request
Contains:CryoStor® CS10

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1 x 106 M34C-GCSF-1
$825.00 Cryopreserved
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5 x 106 M34C-GCSF-2
$2,950.00 Cryopreserved
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10 x 106 M34C-GCSF-3
$4,300.00 Cryopreserved
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25 x 106 M34C-GCSF-4
$6,800.00 Cryopreserved
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50 x 106 M34F-GCSF-5
Inquire Fresh
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Description

Product Overview:

CD34+ stem cells are multipotent and can give rise to all cell types in blood. CD34 cells are most known for its expression on hematopoietic progenitor cells found in bone marrow and cord blood. While compromising only 1.5% of bone marrow mononuclear cell population, CD34 stem cells are responsible for all lymphohematopoietic lineages and are now commonly used clinically as they have been shown to provide long-term durable donor-derived host lymphohematopoietic reconstitution.

Donors are injected with G-CSF (Neupogen® – FDA approved), which stimulates the bone marrow to produce a large number of hematopoietic and progenitor stem cells and mobilizes them into the peripheral blood stream.

CD34+ cells are isolated using positive immunomagnetic cell separation procedures from a mobilized leukapheresis collection.

Storage Conditions:

Use fresh mobilized peripheral blood CD34+ stem/progenitor cells immediately upon receipt.

For cryopreserved mobilized peripheral blood CD34+ stem/progenitor cells, either prepare cells for long-term storage in Liquid Nitrogen vapor phase or thaw for use. Storage in liquid phase nitrogen is NOT recommended. Short-term storage of cells (less than 2 weeks) at -80°C is acceptable but should be minimized to ensure maximum stability. Once thawed, samples must be used immediately.

Testing: Donors are tested for HBV, HCV, HIV, HTLV, WNV, Trypanasoma cruzi, and Syphilis.

Mobilized Peripheral Blood CD34+ Stem/Progenitor Cells Flow Data

CD45 versus PI flow data
CD45 and Propidium Iodide (PI) are used to assess purity and viability of PBMCs. CD45 is expressed on all leukocytes and is used to indicate purity of the white blood cell (WBC) component. Because PI cannot pass through the membrane of live cells to stain DNA, it serves as an indicator of viability. Representative data shown here indicates purity of 95.09% – meaning >95% of cells in this sample are WBCs. (May not be indicative of received samples.)

CD45 versus CD34 flow data
The CD34 protein is a transmembrane protein expressed on early hematopoietic and vascular-associated tissues. It is the most frequently used cell surface marker for stem cells. Side scatter (SSC) is a measurement of inner cellular complexity. CD34+, when assessed in the CD45+ cell population, is a measure of stem cell purity in the enriched sample. This graph shows a representative data set with stem cell purity of 97.62%. (Representative data only. Actual purity percentages will vary between samples.)

Certificate of Analysis

Cryopreserved Mobilized CD34 Stem Cells – GCSF Only
Fresh Mobilized CD34 Stem Cells – GCSF Only

Protocols

How to Thaw Cryopreserved Cells
How to Cryopreserve Human Primary Cells
How to Remove HypoThermosol® from Fresh Cells