CD34+ stem cells are multipotent and can give rise to all cell types in blood. CD34 cells are most known for its expression on hematopoietic progenitor cells found in bone marrow and cord blood. While compromising only 1.5% of bone marrow mononuclear cell population, CD34 stem cells are responsible for all lymphohematopoietic lineages and are now commonly used clinically as they have been shown to provide long-term durable donor-derived host lymphohematopoietic reconstitution.
Donors are injected with G-CSF (Neupogen® – FDA approved), which stimulates the bone marrow to produce a large number of hematopoietic and progenitor stem cells and mobilizes them into the peripheral blood stream.
CD34+ cells are isolated using positive immunomagnetic cell separation procedures from a mobilized leukapheresis collection.
Use fresh mobilized peripheral blood CD34+ stem/progenitor cells immediately upon receipt.
For cryopreserved mobilized peripheral blood CD34+ stem/progenitor cells, either prepare cells for long-term storage in Liquid Nitrogen vapor phase or thaw for use. Storage in liquid phase nitrogen is NOT recommended. Short-term storage of cells (less than 2 weeks) at -80°C is acceptable but should be minimized to ensure maximum stability. Once thawed, samples must be used immediately.
Testing: Donors are tested for HBV, HCV, HIV, HTLV, WNV, Trypanosoma cruzi, and Syphilis.