A mononuclear cell is any cell that has a single round nucleus; examples of a mononuclear cell are lymphocytes, monocytes, and dendritic cells. These cells are critical components of the immune system which are involved in both humoral and cell-mediated immunity. The mononuclear cell is a widely used product in research and clinical applications such as in microbiology, virology, oncology, vaccine development, transplant and regenerative biology, and toxicology.
CD34 depleted mononuclear cells from mobilized peripheral blood are isolated by depleting the CD34 cells using immunomagnetic anti-CD34 microbeads.
For cryopreserved mobilized peripheral blood CD34 depleted mononuclear cells, either prepare cells for long-term storage in Liquid Nitrogen vapor phase or thaw for use. Storage in liquid phase nitrogen is NOT recommended. Short-term storage of cells (less than 2 weeks) at -80°C is acceptable but should be minimized to ensure maximum stability. Once thawed, samples must be used immediately.
Testing: Donors are tested for HBV, HCV, HIV, HTLV, WNV, Trypanosoma cruzi, and Syphilis.