Mobilized Peripheral Blood CD133+ Stem/Progenitor Cells

$0.00$5,600.00

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Purity:

Viability:

Contains:Autologous plasma, HypoThermosol®, or customer specific media upon request
Contains:CryoStor® CS10

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5 x 106 M133F-3
Inquire Fresh
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1 x 106 M133C-1
$1,600.00 Cryopreserved
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2.5 x 106 M133C-2
$3,200.00 Cryopreserved
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5 x 106 M133C-3
$5,600.00 Cryopreserved
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Description

Product Overview:

Cells expressing the CD133 (AC133, Prominin-1) marker are generally known as primitive hematopoietic stem and progenitor cells (HSC and HPC). While cells expressing the CD133 marker are usually undifferentiated embryonic stem cell lines, this cell type is usually harvested from bone marrow and cord blood but can be also found in multiple locations including the liver, muscle, prostate, kidney, and neural tissue. CD133 stem cells appear to be ancestral to the CD34+ stem cell line due to their ability to differentiate into CD34+ cells and also their higher level of proliferation activity.

Capable of a high level of proliferation and a wide range of differentiation, transplantation of CD133+ cell lines has been shown to not only induced multilineage human hematopoiesis through the CD34+ lineage, but also induce regeneration of cell lines in vitro in multiple locations by differentiating into endothelial cells, neural cells, hepatocytes, osteoblasts, and myocytes, with the list of tissue types this cell can differentiate into gradually increasing.

CD133+ stem cells are isolated using positive immunomagnetic cell separation procedures from a mobilized leukapheresis collection.

Storage Conditions:

Use fresh mobilized CD133+ stem cells immediately upon receipt. Be sure to remove HypoThermosol® prior to use. If not used immediately, temporarily store cells at 0-4°C or freeze for long-term storage.

For cryopreserved mobilized CD133+ stem cells, either prepare cells for long-term storage in Liquid Nitrogen vapor phase or thaw for use. Storage in liquid phase nitrogen is NOT recommended. Short-term storage of cells (less than 2 weeks) at -80°C is acceptable but should be minimized to ensure maximum stability. Once thawed, samples must be used immediately.

Testing: Donors are tested for HBV, HCV, HIV, HTLV, WNV, Trypanasoma cruzi, and Syphilis.

Mobilized Peripheral Blood CD133+ Stem/Progenitor Cells Flow Data

CD45 versus PI flow data
CD45 and Propidium Iodide (PI) are used to assess purity and viability of PBMCs. CD45 is expressed on all leukocytes and is used to indicate purity of the white blood cell (WBC) component. Because PI cannot pass through the membrane of live cells to stain DNA, it serves as an indicator of viability. Representative data shown here indicates purity of 95.09% – meaning >95% of cells in this sample are WBCs. (May not be indicative of received samples.)

CD45 versus CD133 flow data
CD133 is associated with stem cells and is considered one of the critical biomarkers for the characterization of progenitor populations. Side scatter (SSC) is a measurement of inner cellular complexity. CD133+, when assessed in the CD45+ cell population, is a measure of stem cell purity in the enriched sample. This graph shows a representative data set with stem cell purity of 97.78%. (Representative data only. Actual purity percentages will vary between samples.)

Certificate of Analysis

Cryopreserved Mobilized Peripheral Blood CD133 Stem Cells

Protocols

How to Thaw Cryopreserved Cells