Leukopaks from mobilized peripheral blood are collected from healthy human donors under IRB approved informed consent by leukapheresis using the Spectra Optia® Apheresis System CMNC collection protocol. Donors are injected with Mozobil®, which stimulates the bone marrow to produce a large number of hematopoietic and progenitor stem cells and mobilizes them into the peripheral blood stream. This mononuclear cell-rich leukapheresis product is extracted using continuous flow centrifugal technology directly into the collection bag.
Mozobil® in combination with G-CSF is also available.
Use fresh mobilized leukopak immediately upon receipt.
For cryopreserved mobilized leukopak, Either prepare cells for long-term storage in Liquid Nitrogen vapor phase or thaw for use. Storage in liquid phase nitrogen is NOT recommended. Short-term storage of cells (less than 2 weeks) at -80°C is acceptable but should be minimized to ensure maximum stability. Once thawed, samples must be used immediately.
Testing: Donors are tested for HBV, HCV, HIV, HTLV, WNV, Trypanasoma cruzi, and Syphilis.
Mobilized Leukopak Flow Data
Representative flow data: Flow data on Certificate of Analysis for fresh mobilized leukopaks available upon request.