Mobilized Leukopak; G-CSF and Mozobil

$48,000.00$91,000.00

Source:

Status:

Viability:>95% by flow cytometry
Contains:Autologous plasma, HypoThermosol®, or customer specific media upon request
Viability:>95% by flow cytometry
Contains:CryoStor® CS10

For a custom order, contact us or click here to customize.

Select Format

Please specify a format to review quotes

1 Bag M001F-GCSF/MOZ-1
Regimen: 5 Days GCSF; Collect Day 6
$48,000.00 Fresh
Add to cart
2 Bags M001F-GCSF/MOZ-2
Regimen: 5 Days GCSF; Collect Days 5 and 6
$65,000.00 Fresh
Add to cart
Full Collection M001CLPGM-1-KIT
Regimen: 5 Days GCSF, Collect Day 6
$67,200.00 Cryopreserved
Add to cart
Full Collection M001CLPGM-2-KIT
Regimen: 5 Days GCSF; Collect Days 5 and 6
$91,000.00 Cryopreserved
Add to cart
Total:
SKU:

Description

Product Overview:

Leukopaks from mobilized peripheral blood are collected from healthy human donors under IRB approved informed consent by leukapheresis using the Spectra Optia® Apheresis System CMNC collection protocol. Donors are injected with a combination of Mozobil® and G-CSF (Neupogen® – FDA approved), which stimulates the bone marrow to produce a large number of hematopoietic and progenitor stem cells and mobilizes them into the peripheral blood stream. This mononuclear cell-rich leukapheresis product is extracted using continuous flow centrifugal technology directly into the collection bag.

Mozobil® only and G-CSF only protocols are also available.

Storage Conditions:

Use fresh mobilized leukopak immediately upon receipt.

For cryopreserved mobilized leukopak, either prepare cells for long-term storage in Liquid Nitrogen vapor phase or thaw for use. Storage in liquid phase nitrogen is NOT recommended. Short-term storage of cells (less than 2 weeks) at -80°C is acceptable but should be minimized to ensure maximum stability. Once thawed, samples must be used immediately.

Testing: Donors are tested for HBV, HCV, HIV, HTLV, WNV, Trypanasoma cruzi, and Syphilis.

Mobilized Leukopak Flow Data

Representative flow data: Flow data on Certificate of Analysis for fresh mobilized leukopaks available upon request.

CD34 versus SSC flow data
The CD34 protein is a transmembrane protein expressed on early hematopoietic and vascular-associated tissues. It is the most frequently used cell surface marker for stem cells. Side scatter (SSC) is a measurement of inner cellular complexity. CD34+ signal and low SSC is used to quantify stem cell populations within a sample. This graph shows a representative data set with a stem cell population of 0.77%. (Representative data only. Actual percentage of CD34+ cells will vary.)

CD45 versus PI flow data
CD45 and Propidium Iodide (PI) are used to assess purity and viability of leukopaks. CD45 is expressed on all leukocytes and is used to indicate purity of the white blood cell component. PI cannot pass through the membrane of live cells to stain DNA, thus, it serves as an indicator of viability. CD45 values in our leukopaks is typically observed to be between 30-60%.

CD45 versus CD3 flow data
CD3 is a multimeric surface molecule expressed commonly by T cell populations. Assessing CD3 versus CD45 helps enumerate what percentage of white blood cells within the sample are T cells. Representative data shown here indicates a relative T cell population of 48.31%. (May not be indicative of received samples.)

CD8 versus CD4 flow data
CD4 and CD8 are co-receptors that can be used to distinguish the two major classes of T cells – helper and cytotoxic, respectively. The ratio of CD4 and CD8 cells can be used as a marker of immune activation and immune senescence. Representative data indicates a typical CD4/CD8 ratio that is around 2 but may vary depending on the health of donor. (May not be indicative of received samples.)

CD45 versus CD14 flow data
CD14 is a marker expressed on multiple cell types, including monocytes, macrophages, and neutrophils. Assessing CD14 versus CD45 within the mononuclear cell component of peripheral blood will enumerate what percentage of the sample are monocytes. Representative data illustrates a relative monocyte population of 24.06%. (May not be indicative of received samples.)

CD45 versus CD19 flow data
CD19 is one of the proteins found in the B-cell co-receptor complex. It can be used as a marker for the identification and quantification of B cells within the sample. Assessing CD19 vs. CD45 will enumerate what percentage of the white blood cells within the sample are B cells. Representative data illustrates a relative B cell population of 12.93%. (May not be indicative of received samples.)

CD45 versus CD56 flow data
CD56 or neural cell adhesion molecule (NCAM) is a homophilic binding glycoprotein expressed on the surface of natural killer (NK) cells. It can be used to identify the relative frequency of NK cells within the white blood cell (WBC) population. Assessing CD56 vs. CD45 will enumerate what percentage of the WBCs within the sample are NK cells. Representative data illustrates a relative NK cell population of 12.34%. (May not be indicative of received samples.)

Certificate of Analysis

Fresh Mobilized Leukopak – GCSF and Mozobil

Protocols

How to Isolate Peripheral Blood Mononuclear Cells (PBMCs) Using a Density Gradient Reagent
How to Cryopreserve Human Primary Cells
How to Remove HypoThermosol® from Fresh Cells
How to Thaw Cells in a Cryopreserved Leukopak