Mesenchymal stem cells (MSCs) are multipotent stromal cells that can differentiate into a variety of cell types including osteoblasts (bone), chondrocytes (cartilage), adipocytes (fat), myocytes (muscle), and neurons (nerve). Because mesenchymal stem cells are undifferentiated cells, they are able to self-renew and have a high proliferative capacity. Mesenchymal stem cells can be isolated from a variety of different tissues, such as bone marrow, adipose tissue, dental pulp, placenta, and umbilical cord blood.
Mesenchymal stem cells (MSC) are cultured from freshly collected adult human bone marrow. MSCs are isolated by adherence from bone marrow mononuclear cells. Adherent cells were cultured in MEM-Alpha, supplemented with L-Glutamine and Human Platelet Lysate.
Bone marrow aspirate is collected from healthy human volunteer donors under IRB approved informed consent and drawn from the posterior iliac crest using a syringe containing anticoagulant.
For cryopreserved mesenchymal stem cells, either prepare cells for long-term storage in Liquid Nitrogen vapor phase or thaw for use. Storage in liquid phase nitrogen is NOT recommended. Short-term storage of cells (less than 2 weeks) at -80°C is acceptable but should be minimized to ensure maximum stability. Once thawed, samples must be used immediately.
Testing: Donors are tested for HBV, HCV, HIV, HTLV, WNV, Trypanosoma cruzi, and Syphilis.