Memory CD8 T Cells; Negative Selection

$1,670.00

Source:

Status:

Contains:

Purity:

Viability:

For a custom order, contact us or click here to customize.

Select Format

Please specify a format to review quotes

5 x 106 PB0845RONC-1
$1,670.00 Cryopreserved
Add to cart
Total:
SKU:

Description

Product Overview:

Memory T cells are “experienced” T cells that lie dormant in tissues and protect the body from the re-emergence of potentially pathogenic or harmful cells (such as bacteria, viruses, cancer cells). Because memory T cells have encountered their antigen in a previous infection, memory T cells can mount a faster and stronger response the second time around.

Memory CD8 T cells are isolated from mononuclear cells by negative selection using immunomagnetic cell separation procedures. Positive selection is also available at your request.

Storage Conditions:

For cryopreserved memory CD8 T cells, either prepare cells for long-term storage in Liquid Nitrogen vapor phase or thaw for use. Storage in liquid phase nitrogen is NOT recommended. Short-term storage of cells (less than 2 weeks) at -80°C is acceptable but should be minimized to ensure maximum stability. Once thawed, samples must be used immediately.

Testing: Donors are tested for HBV, HCV, HIV, HTLV, WNV, Trypanasoma cruzi, and Syphilis.

Memory CD8 T Cells Flow Data

CD45 versus PI flow data
CD45 and Propidium Iodide (PI) are used to assess purity and viability of PBMCs. CD45 is expressed on all leukocytes and is used to indicate purity of the white blood cell (WBC) component. Because PI cannot pass through the membrane of live cells to stain DNA, it serves as an indicator of viability. Representative data shown here indicates purity of 95.09% – meaning >95% of cells in this sample are WBCs. (May not be indicative of received samples.)

CD45 versus CD3 flow data
Assessing CD3 expression versus CD45 in the isolated cell sample elucidates the purity of the sample for CD3+ T cells. This graph shows a representative data set with purity of 96.75% after isolation. (Actual purity may vary somewhat from sample to sample.)

CD45 versus CD8 flow data
This graph illustrates the purity of isolated CD8+ T cells. Representative data shows a purity of 96.54%.

CD45 versus CD45RO flow data
After antigen experience, memory T cells gain expression of CD45RO. This marker is commonly used to identify memory populations. Assessing CD45RO versus CD45 helps to assess the purity of the isolation procedure for memory cells. This graph shows a representative data set with purity of 97.83%. (Actual purity may vary somewhat from sample to sample.)

CD8 versus CD45RO flow data
After antigen experience, memory T cells gain expression of CD45RO. This marker is commonly used to identify memory populations. Assessing CD45RO versus CD8, a T cell co-receptor specific for cytotoxic T cells, helps to assess the purity and specificity of the isolated cell population. This graph shows a representative data set with purity of 97.65%. (Actual purity may vary somewhat from sample to sample.)

Certificate of Analysis

Cryopreserved Peripheral Blood Memory CD8 T Cells

Protocols

How to Thaw Cryopreserved Cells