M2 Macrophages (Monocyte Derived)

$0.00$1,640.00

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Purity:

Viability:

Contains:Autologous plasma, HypoThermosol®, or customer specific media upon request
Contains:CryoStor® CS10

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2 x 106 PBM2C-MON-1
$910.00 Cryopreserved
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5 x 106 PBM2C-MON-2
$1,210.00 Cryopreserved
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10 x 106 PBM2C-MON-3
$1,640.00 Cryopreserved
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10 x 106 PBM2F-MON-3
Inquire Fresh
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Description

Product Overview:

Macrophages are a type of white blood cell found in all tissues patrolling for potential pathogens. Macrophages are key players in the immune response, basically ridding the body of worn-out cells, foreign substances, microbes, and cancer cells and alerting the immune system to the presence of invaders. Because macrophages possess the ability to eliminate pathogens and recruit and instruct other immune cells, they play a central role in protecting the body and contribute to the pathogenesis of inflammatory and degenerative diseases. Macrophages can modify themselves and change their functional phenotype depending on the environmental cues.

M2 macrophages are derived from purified CD14+ monocytes. CD14+ monocytes are positively selected via immunomagnetic beads and cultured in the presence of M-CSF for 10 days. The M2 phenotyping is confirmed by morphology and the expression of specific biomarkers monitored by flow cytometry. The cells are harvested after 10 days of in vitro culture.

Storage Conditions:

Use fresh M2 macrophages immediately upon receipt. Be sure to remove HypoThermosol® prior to use. If not used immediately, temporarily store cells at 0-4°C or freeze for long-term storage.

For cryopreserved M2 macrophages, either prepare cells for long-term storage in Liquid Nitrogen vapor phase or thaw for use. Storage in liquid phase nitrogen is NOT recommended. Short-term storage of cells (less than 2 weeks) at -80°C is acceptable but should be minimized to ensure maximum stability. Once thawed, samples must be used immediately.

Testing: Donors are tested for HBV, HCV, HIV, HTLV, WNV, Trypanasoma cruzi, and Syphilis.

Monocyte-derived M2 Macrophages Flow Data

CD45 versus PI flow data
CD45 and Propidium Iodide (PI) are used to assess purity and viability of PBMCs. CD45 is expressed on all leukocytes and is used to indicate purity of the white blood cell (WBC) component. Because PI cannot pass through the membrane of live cells to stain DNA, it serves as an indicator of viability. Representative data shown here indicates purity of 95.09% – meaning >95% of cells in this sample are WBCs. (May not be indicative of received samples.)

CD45 versus CD11b flow data
CD11b is surface protein involved in adhesion between a number of cells and is expressed on the surface of monocytes, macrophages, and granulocytes. When comparing levels of CD11b vs. expression of CD45, the purity of the sample for macrophages can be assessed. This graph shows a representative data set with purity of 99.90% after differentiation in culture. (Actual purity may vary somewhat from sample to sample.)

Certificate of Analysis

Cryopreserved Peripheral Blood M2 Macrophages

Protocols

How to Thaw Cryopreserved Cells