Macrophages are a type of white blood cell found in all tissues patrolling for potential pathogens. Macrophages are key players in the immune response, basically ridding the body of worn-out cells, foreign substances, microbes, and cancer cells and alerting the immune system to the presence of invaders. Because macrophages possess the ability to eliminate pathogens and recruit and instruct other immune cells, they play a central role in protecting the body and contribute to the pathogenesis of inflammatory and degenerative diseases. Macrophages can modify themselves and change their functional phenotype depending on the environmental cues.
M2 macrophages are derived from purified CD14+ monocytes. CD14+ monocytes are positively selected via immunomagnetic beads and cultured in the presence of M-CSF for 10 days. The M2 phenotyping is confirmed by morphology and the expression of specific biomarkers monitored by flow cytometry. The cells are harvested after 10 days of in vitro culture.
Use fresh M2 macrophages immediately upon receipt. Be sure to remove HypoThermosol® prior to use. If not used immediately, temporarily store cells at 0-4°C or freeze for long-term storage.
For cryopreserved M2 macrophages, either prepare cells for long-term storage in Liquid Nitrogen vapor phase or thaw for use. Storage in liquid phase nitrogen is NOT recommended. Short-term storage of cells (less than 2 weeks) at -80°C is acceptable but should be minimized to ensure maximum stability. Once thawed, samples must be used immediately.
Testing: Donors are tested for HBV, HCV, HIV, HTLV, WNV, Trypanasoma cruzi, and Syphilis.