Dendritic Cells

$730.00

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2 x 106 PB010C-1
$730.00 Cryopreserved
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Description

Product Overview:

Dendritic cells are highly specialized antigen-processing and antigen-presenting cells that are located in mucosal tissues, skin, and lymphoid tissues. They are also called accessory cells.

Dendritic cells play a major role in the initiation of immune responses as they act as messengers between the innate and adaptive immune systems. The main function of dendritic cells is to process and present antigens on the cell surface of T cells. In addition, dendritic cells contribute to the maintenance of B cell function and recall responses.

Dendritic cells have been used to develop in vitro assay methods for immunological reactions including T-cell stimulation, antigen presentation, contact sensitization, and more.

Dendritic cells are derived from purified monocytes. CD14 monocytes are positively selected and are cultured in the presence of GM-CSF and IL-4 for 7 days; and then harvested.

Storage Conditions:

For cryopreserved dendritic cells, either prepare cells for long-term storage in Liquid Nitrogen vapor phase or thaw for use. Storage in liquid phase nitrogen is NOT recommended. Short-term storage of cells (less than 2 weeks) at -80°C is acceptable but should be minimized to ensure maximum stability. Once thawed, samples must be used immediately.

Testing: Donors are tested for HBV, HCV, HIV, HTLV, WNV, Trypanasoma cruzi, and Syphilis.

Dendritic Cells Flow Data

CD45 versus PI flow data
CD45 and Propidium Iodide (PI) are used to assess purity and viability of PBMCs. CD45 is expressed on all leukocytes and is used to indicate purity of the white blood cell (WBC) component. Because PI cannot pass through the membrane of live cells to stain DNA, it serves as an indicator of viability. Representative data shown here indicates purity of 95.09% – meaning >95% of cells in this sample are WBCs. (May not be indicative of received samples.)

CD45 versus CD1c flow data
CD1c is a glycoprotein that is highly expressed on the surface of monocyte-derived immature dendritic cells. When comparing levels of CD1c versus expression of CD45, the purity of the sample for dendritic cells can be assessed. This graph shows a representative data set with purity of 99.88% after differentiation in culture. (Actual purity may vary somewhat from sample to sample.)

Certificate of Analysis

Cryopreserved Peripheral Blood Dendritic Cells

Protocols

How to Thaw Cryopreserved Cells