CryoStor® CS5 Freeze Media

$340.00

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100 mL Bottle 205102
$340.00 Media
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Description

Product Overview:

CryoStor Pre-formulated with 5% DMSO, CryoStor CS5 routinely outperforms competing freeze media containing 10% DMSO and is recommended for cryopreservation of most cell types.

CryoStor cryopreservation freeze media products have been designed to mitigate temperature-induced molecular cell stress responses during freezing and thawing. CryoStor proprietary freeze media products are intended for cryopreservation of biologics at -80 to -196°C and are based on the novel HypoThermosol formula. All CryoStor products are pre-formulated with USP grade DMSO, a permeant solute cryoprotective agent which helps mitigate damage from the formation of intracellular ice.

Across a broad spectrum of cell types, CryoStor products have proven much more effective in reducing post-preservation necrosis and apoptosis as compared to commercial and home-brew isotonic and extracellular formulations. This enables greatly improved post-thaw cell yield, viability, and recovery.

Offered in 100 mL bottles, CryoStor CS5 meets USP <71> Sterility and USP <85> Endotoxin testing standards, and is manufactured under cGMP.

CryoStor® CS5 Performance Evidence

CryoStor vs home brew media
This chart displays the recovery of Human Mesenchymal Stem Cells Following Cryopreservation With Serum-Free CryoStor® compared to serum containing home-brew media.

Post cryopreservation morphology of hMSC - CryoStor CS5
Representative fluorescent micrographs illustrating post-cryopreservation morphology of hMSC stored in serum-free CryoStor CS5 (green: actin cytoskeleton phalloidin – FITC; red: mitochondria activation – MitoTracker® Red; blue: nuclear stain – Hoechst)

Post cryopreservation morphology of hMSC - FGM + 5% DMSO
Representative fluorescent micrographs illustrating post-cryopreservation morphology of hMSC stored in FGM + 5% DMSO (green: actin cytoskeleton phalloidin – FITC; red: mitochondria activation – MitoTracker® Red; blue: nuclear stain – Hoechst)

Protocols

How to Cryopreserve Human Primary Cells