CD8+ Cytotoxic T Cells

$470.00$740.00

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5 x 106 PB08C-1
$470.00 Cryopreserved
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10 x106 PB08C-2
$740.00 Cryopreserved
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Description

Product Overview:

CD8+ cytotoxic T cells fulfill the role dual role of regulating and/or implementing the body’s immune response by eliminating intracellular pathogens such as viruses, and some bacteria and parasites. Upon recognition of an infected cell, the CD8+ T cell initiates apoptosis through the targeted release of effector proteins contained specialized lytic granules within the T cell.

CD8+ cytotoxic T cells are isolated from leukopaks by positive selection using immunomagnetic cell separation procedures. Negative selection is also available at your request.

Storage Conditions:

For cryopreserved CD8+ cytotoxic T cells, either prepare cells for long-term storage in Liquid Nitrogen vapor phase or thaw for use. Storage in liquid phase nitrogen is NOT recommended. Short-term storage of cells (less than 2 weeks) at -80°C is acceptable but should be minimized to ensure maximum stability. Once thawed, samples must be used immediately.

Testing: Donors are tested for HBV, HCV, HIV, HTLV, WNV, Trypanasoma cruzi, and Syphilis.

CD8+ Cytotoxic T Cells Flow Data

CD45 versus PI flow data
CD45 and Propidium Iodide (PI) are used to assess purity and viability of PBMCs. CD45 is expressed on all leukocytes and is used to indicate purity of the white blood cell (WBC) component. Because PI cannot pass through the membrane of live cells to stain DNA, it serves as an indicator of viability. Representative data shown here indicates purity of 95.09% – meaning >95% of cells in this sample are WBCs. (May not be indicative of received samples.)

CD45 versus CD3 flow data
Assessing CD3 expression versus CD45 in the isolated cell sample elucidates the purity of the sample for CD3+ T cells. This graph shows a representative data set with purity of 96.75% after isolation. (Actual purity may vary somewhat from sample to sample.)

CD45 versus CD8 flow data
This graph illustrates the purity of isolated CD8+ T cells. Representative data shows a purity of 96.54%.

Certificate of Analysis

Cryopreserved Peripheral Blood CD8 T Cells

Protocols

How to Thaw Cryopreserved Cells