CD56+ NK Cells; Negative Selection

$810.00$1,550.00

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5 x 106 PB56NC-1
$810.00 Cryopreserved
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10 x 106 PB56NC-2
$1,550.00 Cryopreserved
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Description

Product Overview:

Natural killer cells (NK cells) are cytotoxic members of the innate immune system. CD56+ NK cells are a major component of the immune system. CD56+ NK cells play a key role in the rejection of tumors and cells infected by viruses; killing by releasing perforin and granzymes that cause the target cell to die by apoptosis.

CD56+ NK cells possess a unique ability to elicit an immune response without the presence of immunoglobulins (Ig) and MHC molecules, but simply through the identification of stressed cells, or through the hypothesized “missing self” in which the NK cell senses the downregulation of the surface MHC molecules which usually accompanies certain intracellular infections.

CD56+ NK cells are isolated from mononuclear cells by negative selection using immunomagnetic cell separation procedures.

Storage Conditions:

For cryopreserved CD56+ NK cells, either prepare cells for long-term storage in Liquid Nitrogen vapor phase or thaw for use. Storage in liquid phase nitrogen is NOT recommended. Short-term storage of cells (less than 2 weeks) at -80°C is acceptable but should be minimized to ensure maximum stability. Once thawed, samples must be used immediately.

Testing: Donors are tested for HBV, HCV, HIV, HTLV, WNV, Trypanasoma cruzi, and Syphilis.

CD56 NK Cells; Negative Selection Flow Data

CD45 versus PI flow data
CD45 and Propidium Iodide (PI) are used to assess purity and viability of PBMCs. CD45 is expressed on all leukocytes and is used to indicate purity of the white blood cell (WBC) component. Because PI cannot pass through the membrane of live cells to stain DNA, it serves as an indicator of viability. Representative data shown here indicates purity of 95.09% – meaning >95% of cells in this sample are WBCs. (May not be indicative of received samples.)

CD45 versus CD56 flow data
CD56 or neural cell adhesion molecule (NCAM) is a homophilic binding glycoprotein expressed on the surface of natural killer (NK) cells. It can be used to identify the relative frequency of NK cells within the white blood cell (WBC) population. Assessing CD56 vs. CD45 will enumerate what percentage of the WBCs within the sample are NK cells. Representative data illustrates a relative NK cell population of 12.34%. (May not be indicative of received samples.)

Assessing CD56 expression versus CD45 in the isolated cell sample elucidates the purity of the sample for CD56+ NK cells. This graph shows a representative data set with purity of 96.64% after isolation. (Actual purity may vary somewhat from sample to sample.)

Certificate of Analysis

Cryopreserved Peripheral Blood CD56 NK Cells – Negative Selected

Protocols

How to Thaw Cryopreserved Cells