CD4+ helper T cells oversee the actions of the human immune system. They are involved in activating and directing other immune cells, specifically: (1) determining B cell antibody class switching, (2) the activation and growth of cytotoxic T cells, and (3) maximizing bactericidal activity of phagocytes (i.e. macrophages).
CD4+ helper T cells are isolated from leukopaks by positive selection using immunomagnetic cell separation procedures. Negative selection is also available at your request.
Fresh CD4 helper T cells must be used immediately upon receipt. Be sure to remove HypoThermosol® prior to use. If not used immediately, temporarily store cells at 0-4°C or freeze for long-term storage.
Cryopreserved CD4 helper T cells must either be prepared for long-term storage in Liquid Nitrogen vapor phase or thaw for use. Storage in liquid phase nitrogen is NOT recommended. Short-term storage of cells (less than 2 weeks) at -80°C is acceptable but should be minimized to ensure maximum stability. Once thawed, samples must be used immediately.
Testing: Donors are tested for HBV, HCV, HIV, HTLV, WNV, Trypanosoma cruzi, and Syphilis.