CD4+ Helper T Cells; Negative Selection

$450.00$1,050.00

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10 x 106 PB04NC-1
$450.00 Cryopreserved
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25 x 106 PB04NC-2
$1,050.00 Cryopreserved
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Description

Product Overview:

CD4+ helper T cells oversee the actions of the human immune system. They are involved in activating and directing other immune cells, specifically: (1) determining B cell antibody class switching, (2) the activation and growth of cytotoxic T cells, and (3) maximizing bactericidal activity of phagocytes (i.e. macrophages).

CD4+ helper T cells are isolated from mononuclear cells by negative selection using immunomagnetic cell separation procedures. Positive selection is also available at your request.

Storage Conditions:

For cryopreserved CD4 helper T cells, either prepare cells for long-term storage in Liquid Nitrogen vapor phase or thaw for use. Storage in liquid phase nitrogen is NOT recommended. Short-term storage of cells (less than 2 weeks) at -80°C is acceptable but should be minimized to ensure maximum stability. Once thawed, samples must be used immediately.

Testing: Donors are tested for HBV, HCV, HIV, HTLV, WNV, Trypanasoma cruzi, and Syphilis.

CD4+ Helper T Cells; Negative Selection Flow Data

CD45 versus PI flow data
CD45 and Propidium Iodide (PI) are used to assess purity and viability of PBMCs. CD45 is expressed on all leukocytes and is used to indicate purity of the white blood cell (WBC) component. Because PI cannot pass through the membrane of live cells to stain DNA, it serves as an indicator of viability. Representative data shown here indicates purity of 95.09% – meaning >95% of cells in this sample are WBCs. (May not be indicative of received samples.)

CD45 versus CD3 flow data
Assessing CD3 expression versus CD45 in the isolated cell sample elucidates the purity of the sample for CD3+ T cells. This graph shows a representative data set with purity of 96.75% after isolation. (Actual purity may vary somewhat from sample to sample.)

CD3 versus CD4 flow data
CD4 is a co-receptor found specifically on helper T cells. Assessing CD4 versus CD3, a T cell specific marker, helps to assess both purity and specificity of the isolation procedure for the CD4 population. This data shows a representative data set with purity of 95.78%. (Actual purity may vary somewhat from sample to sample.)

Certificate of Analysis

Cryopreserved Peripheral Blood CD4 T Cells – Negative Selected

Protocols

How to Thaw Cryopreserved Cells