CD4+/CD25+/CD127- regulatory T cells (or CD127 dim regulatory T cells) express intracellular transcription factor FoxP3. They are critical to the maintenance of immune cell homeostasis and peripheral tolerance. A precise understanding of the immunosuppressive mechanism of regulatory T cells remain elusive, including the secretion of immunosuppressive soluble factors such as IL-9, IL-10 and TGF beta, cell contact mediated regulation via the high-affinity TCR, and other costimulatory molecules such as CTLA-4, GITR, and cytolytic activity. Understanding the immunosuppressive mechanism of regulatory T cells has broad implications for the development of therapeutic strategies for many disease processes including cancer, diabetes, and immune-mediated diseases.
Regulatory T cells are isolated from leukapheresis product by CD127 depletion and positive selection using CD25 immunomagnetic beads.
For cryopreserved regulatory T cells, either prepare cells for long-term storage in Liquid Nitrogen vapor phase or thaw for use. Storage in liquid phase nitrogen is NOT recommended. Short-term storage of cells (less than 2 weeks) at -80°C is acceptable but should be minimized to ensure maximum stability. Once thawed, samples must be used immediately.
Testing: Donors are tested for HBV, HCV, HIV, HTLV, WNV, Trypanasoma cruzi, and Syphilis.