CD4+/CD25+/CD127- Regulatory T Cells



Peripheral Blood




CryoStor® CS10


>80% by flow cytometry


>95% by flow cytometry

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1 x 106 PB425/127NC-1
$3,000.00 Cryopreserved
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2 x 106 PB425/127NC-2
$5,000.00 Cryopreserved
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Product Overview:

CD4+/CD25+/CD127- regulatory T cells (or CD127 dim regulatory T cells) express intracellular transcription factor FoxP3. They are critical to the maintenance of immune cell homeostasis and peripheral tolerance. A precise understanding of the immunosuppressive mechanism of regulatory T cells remain elusive, including the secretion of immunosuppressive soluble factors such as IL-9, IL-10 and TGF beta, cell contact mediated regulation via the high-affinity TCR, and other costimulatory molecules such as CTLA-4, GITR, and cytolytic activity. Understanding the immunosuppressive mechanism of regulatory T cells has broad implications for the development of therapeutic strategies for many disease processes including cancer, diabetes, and immune-mediated diseases.

Regulatory T cells are isolated from leukapheresis product by CD127 depletion and positive selection using CD25 immunomagnetic beads.

Storage Conditions:

For cryopreserved regulatory T cells, either prepare cells for long-term storage in Liquid Nitrogen vapor phase or thaw for use. Storage in liquid phase nitrogen is NOT recommended. Short-term storage of cells (less than 2 weeks) at -80°C is acceptable but should be minimized to ensure maximum stability. Once thawed, samples must be used immediately.

Testing: Donors are tested for HBV, HCV, HIV, HTLV, WNV, Trypanosoma cruzi, and Syphilis.

Regulatory T Cells Flow Data

CD45 versus PI flow data
CD45 and Propidium Iodide (PI) are used to assess purity and viability of PBMCs. CD45 is expressed on all leukocytes and is used to indicate purity of the white blood cell (WBC) component. Because PI cannot pass through the membrane of live cells to stain DNA, it serves as an indicator of viability. Representative data shown here indicates purity of 95.09% – meaning >95% of cells in this sample are WBCs. (May not be indicative of received samples.)

CD3 versus CD4 flow data
CD4 is a co-receptor found specifically on helper T cells. Assessing CD4 versus CD3, a T cell specific marker, helps to assess both purity and specificity of the isolation procedure for the CD4 population. This data shows a representative data set with purity of 95.78%. (Actual purity may vary somewhat from sample to sample.)

FoxP3 marker flow data
FoxP3 is a gene transcription factor which is used as the de facto marker for regulatory T cells in non-activated populations. This representative data illustrates the purity (90.67%) of regulatory T cells after isolation. (Exact purity values may vary from sample to sample.)

Certificate of Analysis

Cryopreserved Peripheral Blood Regulatory T Cells


How to Thaw Cryopreserved Cells