The CD3+ T cell possesses a complex of cell surface molecules that associates with the T-cell antigen receptor (TCR). CD3 functions in the cell surface expression of TCR and in the signaling transduction cascade that originates when a peptide: MHC ligand binds to the TCR. CD3 surface protein is made of invariant subchains belonging to the immunoglobulin superfamily. CD3 is found on the surfaces of T cells and of thymocytes. Mutant expression of the TCR can be found in Severe Combined Immunodeficiency (SCID), and monoclonal antibodies to the CD3 marker have been demonstrated to be an effective way to control allografts by preventing T-cell activation.
CD3+ T cells are isolated from leukopaks by negative selection using immunomagnetic cell separation procedures. Positive selection is also available at your request.
For cryopreserved CD3+ pan T cells; negative selection, either prepare cells for long-term storage in Liquid Nitrogen vapor phase or thaw for use. Storage in liquid phase nitrogen is NOT recommended. Short-term storage of cells (less than 2 weeks) at -80°C is acceptable but should be minimized to ensure maximum stability. Once thawed, samples must be used immediately.
Testing: Donors are tested for HBV, HCV, HIV, HTLV, WNV, Trypanasoma cruzi, and Syphilis.