CD3+ Pan T Cells; Negative Selection

$450.00$1,780.00

Source:

Status:

Contains:

Purity:

Viability:

For a custom order, contact us or click here to customize.

Select Format

Please specify a format to review quotes

5 x 106 PB03NC-1
$450.00 Cryopreserved
Add to cart
10 x 106 PB03NC-2
$690.00 Cryopreserved
Add to cart
25 x 106 PB03NC-3
$1,050.00 Cryopreserved
Add to cart
50 x 106 PB03NC-4
$1,780.00 Cryopreserved
Add to cart
Total:
SKU:

Description

Product Overview:

The CD3+ T cell possesses a complex of cell surface molecules that associates with the T-cell antigen receptor (TCR). CD3 functions in the cell surface expression of TCR and in the signaling transduction cascade that originates when a peptide: MHC ligand binds to the TCR. CD3 surface protein is made of invariant subchains belonging to the immunoglobulin superfamily. CD3 is found on the surfaces of T cells and of thymocytes. Mutant expression of the TCR can be found in Severe Combined Immunodeficiency (SCID), and monoclonal antibodies to the CD3 marker have been demonstrated to be an effective way to control allografts by preventing T-cell activation.

CD3+ T cells are isolated from mononuclear cells by negative selection using immunomagnetic cell separation procedures. Positive selection is also available at your request.

Storage Conditions:

For cryopreserved CD3+ pan T cells; negative selection, either prepare cells for long-term storage in Liquid Nitrogen vapor phase or thaw for use. Storage in liquid phase nitrogen is NOT recommended. Short-term storage of cells (less than 2 weeks) at -80°C is acceptable but should be minimized to ensure maximum stability. Once thawed, samples must be used immediately.

Testing: Donors are tested for HBV, HCV, HIV, HTLV, WNV, Trypanasoma cruzi, and Syphilis.

CD3+ Pan T Cells; Negative Selection Flow Data

CD45 versus PI flow data
CD45 and Propidium Iodide (PI) are used to assess purity and viability of PBMCs. CD45 is expressed on all leukocytes and is used to indicate purity of the white blood cell (WBC) component. Because PI cannot pass through the membrane of live cells to stain DNA, it serves as an indicator of viability. Representative data shown here indicates purity of 95.09% – meaning >95% of cells in this sample are WBCs. (May not be indicative of received samples.)

CD45 versus CD3 flow data
Assessing CD3 expression versus CD45 in the isolated cell sample elucidates the purity of the sample for CD3+ T cells. This graph shows a representative data set with purity of 96.75% after isolation. (Actual purity may vary somewhat from sample to sample.)

CD8 versus CD4 flow data
CD4 and CD8 are co-receptors that can be used to distinguish the two major classes of T cells – helper and cytotoxic, respectively. The ratio of CD4 and CD8 cells can be used as a marker of immune activation and immune senescence. Representative data indicates a typical CD4/CD8 ratio that is around 2 but may vary depending on the health of donor. (May not be indicative of received samples.)

Certificate of Analysis

Cryopreserved Peripheral Blood CD3 T Cells – Negative Selected

Protocols

How to Thaw Cryopreserved Cells