CD3+ Gamma/Delta T Cells; Negative Selection

$2,925.00

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10 x 106 PB03NC-TCRG/D-1
$2,925.00 Cryopreserved
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Description

Product Overview:

Gamma delta T cells (γδ T cells) express a unique T-cell receptor (TCR) composed of one γ-chain and one δ-chain. Gamma delta T cells are in low abundance in the body and are found in the gut mucosa, skin, lungs, and uterus. These cells are involved in the initiation and propagation of immune responses. They are considered to represent a link between innate and adaptive immunity. They can secrete particular effector cytokines in a subtype- and context-specific manner and are capable of phagocytosis.

CD3+ T-cell receptor γ/δ+ (TCRγ/δ+) cells are negatively selected via immunomagnetic bead separation from a leukapheresis collection.

Storage Conditions:

For cryopreserved gamma delta T cells, either prepare cells for long-term storage in Liquid Nitrogen vapor phase or thaw for use. Storage in liquid phase nitrogen is NOT recommended. Short-term storage of cells (less than 2 weeks) at -80°C is acceptable but should be minimized to ensure maximum stability. Once thawed, samples must be used immediately.

Testing: Donors are tested for HBV, HCV, HIV, HTLV, WNV, Trypanasoma cruzi, and Syphilis.

CD3+ Gamma/Delta T Cells Flow Data

CD45 versus PI flow data
CD45 and Propidium Iodide (PI) are used to assess purity and viability of PBMCs. CD45 is expressed on all leukocytes and is used to indicate purity of the white blood cell (WBC) component. Because PI cannot pass through the membrane of live cells to stain DNA, it serves as an indicator of viability. Representative data shown here indicates purity of 95.09% – meaning >95% of cells in this sample are WBCs. (May not be indicative of received samples.)

CD45 versus CD3 flow data
Assessing CD3 expression versus CD45 in the isolated cell sample elucidates the purity of the sample for CD3+ T cells. This graph shows a representative data set with purity of 96.75% after isolation. (Actual purity may vary somewhat from sample to sample.)

CD45 versus GDT flow data
Gamma delta T cells are the counterpart to the more common alpha beta T cells and represent a small subset of T cells in peripheral blood. This graph shows a representative data set with purity of 93.58% after isolation. (Actual purity may vary somewhat from sample to sample.)

Certificate of Analysis

Cryopreserved Peripheral Blood Gamma Delta T Cells

Protocols

How to Thaw Cryopreserved Cells