Gamma delta T cells (γδ T cells) express a unique T-cell receptor (TCR) composed of one γ-chain and one δ-chain. Gamma delta T cells are in low abundance in the body and are found in the gut mucosa, skin, lungs, and uterus. These cells are involved in the initiation and propagation of immune responses. They are considered to represent a link between innate and adaptive immunity. They can secrete particular effector cytokines in a subtype- and context-specific manner and are capable of phagocytosis.
CD3+ T-cell receptor γ/δ+ (TCRγ/δ+) cells are negatively selected via immunomagnetic bead separation from a leukapheresis collection.
For cryopreserved gamma delta T cells, either prepare cells for long-term storage in Liquid Nitrogen vapor phase or thaw for use. Storage in liquid phase nitrogen is NOT recommended. Short-term storage of cells (less than 2 weeks) at -80°C is acceptable but should be minimized to ensure maximum stability. Once thawed, samples must be used immediately.
Testing: Donors are tested for HBV, HCV, HIV, HTLV, WNV, Trypanasoma cruzi, and Syphilis.