CD19+ B Cells

$495.00$1,470.00

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5 x 106 PB19C-1
$495.00 Cryopreserved
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10 x 106 PB19C-2
$750.00 Cryopreserved
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25 x 106 PB19C-3
$1,470.00 Cryopreserved
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Description

Product Overview:

CD19 B cells play an essential role in the adaptive immune response. CD19+ B cells are responsible for the recognition and clearance of antigens in the system and, in the process, creating cells with the purpose of immunosurveillance. The wide range and sensitivity of the B cell to antigens lies in the process in which its receptor, the B cell receptor, develops.

CD19 B cells are isolated from mononuclear cells by positive selection using immunomagnetic cell separation procedures. Negative selection is also available at your request.

Storage Conditions:

For cryopreserved CD19+ B cells, either prepare cells for long-term storage in Liquid Nitrogen vapor phase or thaw for use. Storage in liquid phase nitrogen is NOT recommended. Short-term storage of cells (less than 2 weeks) at -80°C is acceptable but should be minimized to ensure maximum stability. Once thawed, samples must be used immediately.

Testing: Donors are tested for HBV, HCV, HIV, HTLV, WNV, Trypanasoma cruzi, and Syphilis.

CD19 B Cells Flow Data

CD45 versus PI flow data
This graph highlights both the purity and viability of the mononuclear cells. CD45 is used to indicate purity of the white blood cells, while PI is used to differentiate live and dead cells. Representative data shows a purity of 95.09%.

CD20 versus CD22 flow data
CD20 and CD22 are co-receptors found on B cells. These are used to quantify the purity of isolated B cells when the CD19 epitope is blocked. This graph shows a representative data set with purity of 97.24% after isolation. (Actual purity may vary somewhat from sample to sample.)

Certificate of Analysis

Cryopreserved Peripheral Blood CD19 B Cells

Protocols

How to Thaw Cryopreserved Cells