CD19+ B Cells; Negative Selection

$840.00

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10 x 106 PB19NC-1
$840.00 Cryopreserved
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Description

Product Overview:

CD19+ B cells play an essential role in the adaptive immune response. CD19+ B cells are responsible for the recognition and clearance of antigens in the system and, in the process, creating cells with the purpose of immunosurveillance. The wide range and sensitivity of the B cell to antigens lies in the process in which its receptor, the B cell receptor, develops.

CD19+ B cells are isolated from mononuclear cells by negative selection using immunomagnetic cell separation procedures. Positive selection is also available at your request.

Storage Conditions:

For cryopreserved CD19+ B cells, either prepare cells for long-term storage in Liquid Nitrogen vapor phase or thaw for use. Storage in liquid phase nitrogen is NOT recommended. Short-term storage of cells (less than 2 weeks) at -80°C is acceptable but should be minimized to ensure maximum stability. Once thawed, samples must be used immediately.

Testing: Donors are tested for HBV, HCV, HIV, HTLV, WNV, Trypanasoma cruzi, and Syphilis.

CD19 B Cells; Negative Selection Flow Data

CD45 versus PI flow data
CD45 and Propidium Iodide (PI) are used to assess purity and viability of PBMCs. CD45 is expressed on all leukocytes and is used to indicate purity of the white blood cell (WBC) component. Because PI cannot pass through the membrane of live cells to stain DNA, it serves as an indicator of viability. Representative data shown here indicates purity of 95.09% – meaning >95% of cells in this sample are WBCs. (May not be indicative of received samples.)

CD45 versus CD19 flow data
Assessing CD19 expression versus CD45 in the isolated cell sample elucidates the purity of the sample for CD19+ B cells. This graph shows a representative data set with purity of 98.38% after isolation. (Actual purity may vary somewhat from sample to sample.)

Certificate of Analysis

Cryopreserved Peripheral Blood CD19 B Cells – Negative Selected

Protocols

How to Thaw Cryopreserved Cells