CD14+ Monocytes; Negative Selection

isolated cells
isolated primary cells
cryopreserved human cells
cryopreserved cells

$810.00 – $1,795.00

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FreshCryopreserved
10 x 106 PB14NC-1
$810.00 Cryopreserved
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25 x 106 PB14NC-2
$1,390.00 Cryopreserved
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50 x 106 PB14NC-3
$1,795.00 Cryopreserved
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Description

Product Overview:

CD14+ monocytes are responsible for phagocytosis of foreign substances in the body and are capable of killing infected host cells via antibody-mediated cellular cytotoxicity. Under the right culture conditions, monocytes can differentiate into dendritic cells, Langerhans cells, or macrophages.

CD14+ monocytes are isolated from mononuclear cells by negative selection using immunomagnetic cell separation procedures. Positive selection is also available at your request.

Storage Conditions:

For cryopreserved CD14+ monocytes, either prepare cells for long-term storage in Liquid Nitrogen vapor phase or thaw for use. Storage in liquid phase nitrogen is NOT recommended. Short-term storage of cells (less than 2 weeks) at -80°C is acceptable but should be minimized to ensure maximum stability. Once thawed, samples must be used immediately.

Testing: Donors are tested for HBV, HCV, HIV, HTLV, WNV, Trypanasoma cruzi, and Syphilis.

CD14 Monocytes; Negative Selection Flow Data

CD45 versus PI flow data
CD45 and Propidium Iodide (PI) are used to assess purity and viability of PBMCs. CD45 is expressed on all leukocytes and is used to indicate purity of the white blood cell (WBC) component. Because PI cannot pass through the membrane of live cells to stain DNA, it serves as an indicator of viability. Representative data shown here indicates purity of 95.09% – meaning >95% of cells in this sample are WBCs. (May not be indicative of received samples.)

CD45 versus CD14 flow data
CD14 is a marker expressed on multiple cell types, including monocytes, macrophages, and neutrophils. Assessing CD14 versus CD45 within the mononuclear cell component of peripheral blood will enumerate what percentage of the sample are monocytes. Representative data illustrates a relative monocyte population of 24.06%. (May not be indicative of received samples.)

CD45 versus CD14 flow data
Assessing CD14 expression versus CD45 in the isolated cell sample elucidates the purity of the sample for CD14+ monocytes. This graph shows a representative data set with purity of 97.80% after isolation. (Actual purity may vary somewhat from sample to sample.)

Certificate of Analysis

Cryopreserved Peripheral Blood CD14 Monocytes – Negative Selected

Protocols

How to Thaw Cryopreserved Cells

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