CD14+ monocytes (classical monocytes) are responsible for phagocytosis of foreign substances in the body and are capable of killing infected host cells via antibody-mediated cellular cytotoxicity. Under the right culture conditions, monocytes can differentiate into dendritic cells, Langerhans cells, or macrophages. CD14+ monocytes are the most common subset of monocytes found in the periphery, and can be characterized by high CD14 expression and low CD16 expression (CD14++, CD16-).
CD14+ monocytes are isolated from mononuclear cells by negative selection using immunomagnetic cell separation procedures. This isolation depletes non-target cells via their lineage surface antigens, and includes reagents which remove the intermediate (CD14+, CD16+) and non-classical (CD14+, CD16++) monocyte subsets. Positive selection is also available at your request.
For cryopreserved CD14+ monocytes, either prepare cells for long-term storage in Liquid Nitrogen vapor phase or thaw for use. Storage in liquid phase nitrogen is NOT recommended. Short-term storage of cells (less than 2 weeks) at -80°C is acceptable but should be minimized to ensure maximum stability. Once thawed, samples must be used immediately.
Testing: Donors are tested for HBV, HCV, HIV, HTLV, WNV, Trypanosoma cruzi, and Syphilis.