Bone Marrow Mononuclear Cells (BMMCs)
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Bone marrow mononuclear cells are isolated from bone marrow aspirate using density gradient centrifugation techniques. Bone marrow aspirates and cells are drawn from the posterior iliac crest using a syringe containing anticoagulant collected from healthy human donors under IRB approved informed consent.
For cryopreserved bone marrow mononuclear cells, either prepare cells for long-term storage in Liquid Nitrogen vapor phase or thaw for use. Storage in liquid phase nitrogen is NOT recommended. Short term storage of cells (less than 2 weeks) at -80°C is acceptable but should be minimized to ensure maximum stability. Once thawed, samples must be used immediately.
Testing: Donors are tested for HBV, HCV, HIV, HTLV, WNV, Trypanasoma cruzi, and Syphilis.
Bone Marrow Mononuclear Cells Flow Data
The CD34 protein is a transmembrane protein expressed on early hematopoietic and vascular-associated tissues. It is the most frequently used cell surface marker for stem cells. Side scatter (SSC) is a measurement of inner cellular complexity. CD34+ signal and low SSC is used to quantify stem cell populations within a sample. This graph shows a representative data set with a stem cell population of 0.77%. (Representative data only. Actual percentage of CD34+ cells will vary.)
CD45 and Propidium Iodide (PI) are used to assess purity and viability of PBMCs. CD45 is expressed on all leukocytes and is used to indicate purity of the white blood cell (WBC) component. Because PI cannot pass through the membrane of live cells to stain DNA, it serves as an indicator of viability. Representative data shown here indicates purity of 95.09% – meaning >95% of cells in this sample are WBCs. (May not be indicative of received samples.)
CD3 is a multimeric surface molecule expressed commonly by T cell populations. Assessing CD3 versus CD45 helps enumerate what percentage of white blood cells within the sample are T cells. Representative data shown here indicates a relative T cell population of 48.31%. (May not be indicative of received samples.)
CD4 and CD8 are co-receptors that can be used to distinguish the two major classes of T cells – helper and cytotoxic, respectively. The ratio of CD4 and CD8 cells can be used as a marker of immune activation and immune senescence. Representative data indicates a typical CD4/CD8 ratio that is around 2 but may vary depending on the health of donor. (May not be indicative of received samples.)
CD14 is a marker expressed on multiple cell types, including monocytes, macrophages, and neutrophils. Assessing CD14 versus CD45 within the mononuclear cell component of peripheral blood will enumerate what percentage of the sample are monocytes. Representative data illustrates a relative monocyte population of 24.06%. (May not be indicative of received samples.)
CD19 is one of the proteins found in the B-cell co-receptor complex. It can be used as a marker for the identification and quantification of B cells within the sample. Assessing CD19 vs. CD45 will enumerate what percentage of the white blood cells within the sample are B cells. Representative data illustrates a relative B cell population of 12.93%. (May not be indicative of received samples.)
CD56 or neural cell adhesion molecule (NCAM) is a homophilic binding glycoprotein expressed on the surface of natural killer (NK) cells. It can be used to identify the relative frequency of NK cells within the white blood cell (WBC) population. Assessing CD56 vs. CD45 will enumerate what percentage of the WBCs within the sample are NK cells. Representative data illustrates a relative NK cell population of 12.34%. (May not be indicative of received samples.)