CD34+ stem cells are multipotent and can give rise to all cell types in blood. CD34 cells are most known for its expression on hematopoietic progenitor cells found in bone marrow and cord blood. While compromising only 1.5% of bone marrow mononuclear cell population, CD34 stem cells are responsible for all lymphohematopoietic lineages and are now commonly used clinically as they have been shown to provide long-term durable donor-derived host lymphohematopoietic reconstitution.
CD34+ cells are isolated using positive immunomagnetic cell separation procedures from bone marrow.
For cryopreserved CD34+ stem cells, either prepare cells for long-term storage in Liquid Nitrogen vapor phase or thaw for use. Storage in liquid phase nitrogen is NOT recommended. Short-term storage of cells (less than 2 weeks) at -80°C is acceptable but should be minimized to ensure maximum stability. Once thawed, samples must be used immediately.
Testing: Donors are tested for HBV, HCV, HIV, HTLV, WNV, Trypanasoma cruzi, and Syphilis.