Bone Marrow CD34 Depleted Mononuclear Cells

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Contains:Autologous plasma, HypoThermosol®, or customer specific media upon request
Contains:CryoStor® CS10

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Description

Product Overview:

Bone Marrow CD34 depleted mononuclear cells (BMMCs) are isolated by depleting the CD34 cells using immunomagnetic anti-CD34 microbeads.

Storage Conditions:

Use fresh CD34 depleted mononuclear cells immediately upon receipt. Be sure to remove HypoThermosol® prior to use. If not used immediately, temporarily store cells at 0-4°C or freeze for long-term storage.

For cryopreserved CD34 depleted mononuclear cells, either prepare cells for long-term storage in Liquid Nitrogen vapor phase or thaw for use. Storage in liquid phase nitrogen is NOT recommended. Short-term storage of cells (less than 2 weeks) at -80°C is acceptable but should be minimized to ensure maximum stability. Once thawed, samples must be used immediately.

Testing: Donors are tested for HBV, HCV, HIV, HTLV, WNV, Trypanasoma cruzi, and Syphilis.

Bone Marrow CD34 Depleted Mononuclear Cells Flow Data

CD45 versus PI flow data
CD45 and Propidium Iodide (PI) are used to assess purity and viability of PBMCs. CD45 is expressed on all leukocytes and is used to indicate purity of the white blood cell (WBC) component. Because PI cannot pass through the membrane of live cells to stain DNA, it serves as an indicator of viability. Representative data shown here indicates purity of 95.09% – meaning >95% of cells in this sample are WBCs. (May not be indicative of received samples.)

CD45 versus CD3 flow data
CD3 is a multimeric surface molecule expressed commonly by T cell populations. Assessing CD3 versus CD45 helps enumerate what percentage of white blood cells within the sample are T cells. Representative data shown here indicates a relative T cell population of 48.31%. (May not be indicative of received samples.)

CD8 versus CD4 flow data
CD4 and CD8 are co-receptors that can be used to distinguish the two major classes of T cells – helper and cytotoxic, respectively. The ratio of CD4 and CD8 cells can be used as a marker of immune activation and immune senescence. Representative data indicates a typical CD4/CD8 ratio that is around 2 but may vary depending on the health of donor. (May not be indicative of received samples.)

CD45 versus CD14 flow data
CD14 is a marker expressed on multiple cell types, including monocytes, macrophages, and neutrophils. Assessing CD14 versus CD45 within the mononuclear cell component of peripheral blood will enumerate what percentage of the sample are monocytes. Representative data illustrates a relative monocyte population of 24.06%. (May not be indicative of received samples.)

CD45 versus CD19 flow data
CD19 is one of the proteins found in the B-cell co-receptor complex. It can be used as a marker for the identification and quantification of B cells within the sample. Assessing CD19 vs. CD45 will enumerate what percentage of the white blood cells within the sample are B cells. Representative data illustrates a relative B cell population of 12.93%. (May not be indicative of received samples.)

CD45 versus CD56 flow data
CD56 or neural cell adhesion molecule (NCAM) is a homophilic binding glycoprotein expressed on the surface of natural killer (NK) cells. It can be used to identify the relative frequency of NK cells within the white blood cell (WBC) population. Assessing CD56 vs. CD45 will enumerate what percentage of the WBCs within the sample are NK cells. Representative data illustrates a relative NK cell population of 12.34%. (May not be indicative of received samples.)

CD34 versus SSC flow data
The depletion of CD34+ stem cells is illustrated in this graph. After processing, a sample with a typically enriched stem cell population (such as cord blood, bone marrow, or mobilized peripheral blood) is depleted, leaving only a fraction of the original population (0.07% in this data set). (May not be indicative of received samples.)

Certificate of Analysis

Cryopreserved Bone Marrow CD34 Depleted Mononuclear Cells
Fresh Bone Marrow CD34 Depleted Mononuclear Cells

Protocols

How to Cryopreserve Human Primary Cells
How to Thaw Cryopreserved Cells
How to Remove HypoThermosol® from Fresh Cells