Anti-SARS-CoV-2 S424-433 T Cells

$650.00

Source:

Peripheral Blood

Status:

Healthy

Viability:

>95% by flow cytometry

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1 x 106 1142
$650.00 Cryopreserved
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Description

Product Overview:

The SARS-CoV-2 antigen-specific T cell is an HLA-A*0201-restricted T cell line with the specificity for the KLPDDFTGCV, a peptide that corresponds to the Spike protein (position 424-433) of the novel coronavirus SARS-CoV-2.

COVID-19 antigen-specific T cells can be utilized for a multitude of applications including examining T cell proliferation, cytokine expression, and cytotoxicity in response to the immuno-modulatory activity of specific anti-COVID-19 drugs. These unique cells provide valuable insight regarding the T cell response to COVID-19 infection, thus accelerating early-stage research, drug discovery, as well as disease diagnosis and treatment.

Storage Conditions:

For cryopreserved antigen-specific T cells, prepare cells for long-term storage in Liquid Nitrogen vapor phase or thaw for use. Storage in liquid phase nitrogen is NOT recommended. Short-term storage of cells (less than 2 weeks) at -80°C is acceptable but should be minimized to ensure maximum stability. Once thawed, samples must be used immediately.

Testing:

Donors are tested for the blood borne pathogens HIV-1 and 2, Hepatitis B, Hepatitis C, and HTLV-1.

Test agent (right dotplot): SARS-CoV-2-S424 Dex = SARS-CoV-2-S424-433 peptide: (KLPDDFTGCV) HLA-A*0201-PE MHC Dextramer (Immudex, Copenhagen, Denmark); control agent (left dotplot) SARS-CoV-2-S1220 Dex = SARS-CoV-2-S1220-1228 peptide: (FIAGLIAIV) HLA-A*0201-PE MHC Dextramer (Immudex, Copenhagen, Denmark))

Functional Assay of anti-SARS-CoV-2-S424-433 T cells (Antigen-Specific IFN-gamma Secretion [left figure] and Cytotoxicity Assay [right figure])

40,000 T cells were plated in a 96-well round-bottom plate in the presence of 40,000 T2 cells, a B-LCL expressing HLA-A*0201) alone or in the presence of increasing concentrations of test peptide: HLA-A*0201 restricted SARS-CoV-2 S424-433 peptide (KLPDDFTGCV) or irrelevant control peptide: HLA-A*0201 restricted SARS-CoV-2 S1220-1228 (FIAGLIAIV). Assay media is RPMI-1640 + 10% FBS + 2 mM L-Glutamine.

Supernatants were collected after 18-24 hours or incubation. IFN-g concentration was analyzed using the Meso Scale Discovery IFN-g assay (left figure).

The remaining cells were labelled with 7-AAD and analyzed for cell viability/death on a flow cytometer. Target cells (T2) were initially gated based on forward and side scatter properties. Percent cytotoxicity, represented by % 7-AAD+ target cells (T2) is reported against peptide concentration (right figure).

Certificate of Analysis

Cryopreserved Anti-SARS-CoV-2 S424-433 T Cells

Protocols

How to Thaw Antigen-Specific T Cells