What is Peripheral Blood?
Peripheral blood, the blood circulating throughout the body, is an important starting material for many scientists. The cellular components of peripheral blood include erythrocytes (red blood cells), leukocytes (white blood cells), and thrombocytes (platelets). Plasma, the liquid component of peripheral blood, is the vehicle through which these blood cells circulate the body.
Why Use Peripheral Blood Products?
Peripheral blood cells are utilized across the research and development spectrum in many branches of science including microbiology, virology, oncology, vaccine development, transplant and regenerative biology, and toxicology. Their popularity in research is due to the fact that the white blood cell component, notably peripheral blood mononuclear cells (PBMCs) which include subsets from the lymphoid lineage (T cells, B cells, NK cells) and myeloid lineage (monocytes, dendritic cells), are critical components of the innate and adaptive immune system.
How are Peripheral Blood Products Collected?
Peripheral blood cells are collected either through traditional venipuncture procedures or apheresis by using the Spectra Optia® Apheresis System. Collection is performed in HemaCare’s FDA-registered donor center from IRB-consented healthy human donors.
Unprocessed peripheral blood products are shipped immediately to customers or processed on-site following collection utilizing best-in-class instruments and media and standardized protocols. Rigorous operational controls ensure customers receive reproducible and consistent high-quality cells, eliminating variability. All isolated primary cells are characterized by flow cytometry prior to cryopreservation, guaranteeing customers receive a pure and viable cell population every time.
What is Apheresis?
Apheresis is the process of removing a specific component from the blood and returning the remaining components to the donor. HemaCare utilizes the Spectra Optia® Apheresis System, an industry-leading cell processing and cell collection platform that uses continuous-flow centrifugation and optical detection technology, to collect white blood cells (this procedure is called “leukapheresis”).
Leukapheresis provides a more concentrated cellular fraction than could be separated from a unit of whole blood via traditional methods. Typically, the mononuclear cell content with leukapheresis is 20 times higher than that obtained from the buffy coat. Due to the large size of the collection and the benefit of obtaining a purer and higher yield of mononuclear cells, products obtained from leukapheresis (leukopaks) are regularly used in both the research setting, and cell therapy process development, as well as clinically for certain treatment procedures