A mononuclear (MNC) cell is any cell that has a single round nucleus; examples of a mononuclear cell are lymphocytes, monocytes, and dendritic cells. These cells are critical components of the immune system which are involved in both humoral and cell-mediated immunity. MNCs are widely used product in research and clinical applications such as in microbiology, virology, oncology, vaccine development, transplant and regenerative biology, and toxicology.
MNCs are obtained using density gradient centrifugation techniques. MNCs can be further processed to isolate a variety of subpopulations and are available in a fresh or cryopreserved format.