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Home > Research Products > Human-Derived Biological Products: Mononuclear Cells

Human-Derived Biological Products

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Mononuclear Cells

  • Description

  • Customized Products

  • Disease-State
    Products

  • Packing & Shipping

  • Donor Criteria

LeukocytesA mononuclear cell is any cell that has a single round nucleus; examples of a mononuclear cell are lymphocytes, monocytes, and dendritic cells.  These cells are critical components of the immune system which are involved in both humoral and cell-mediated immunity.  The mononuclear cell is a widely used product in research and clinical applications such as in microbiology, virology, oncology, vaccine development, transplant and regenerative biology, and toxicology.

 

Peripheral Blood Mononuclear Cells (PBMC)

Peripheral blood mononuclear cells (PBMC) are collected in HemaCare's FDA-registered collection centers following cGMP and cGTP collection guidelines from IRB consented healthy human volunteer donors  by leukapheresis using the COBE® Spectra Apheresis System MNC collection protocol.  Peripheral blood mononuclear cells (PBMC) are obtained from a leukapheresis product purified by a density gradient centrifugation and are available in fresh or cryopreserved format.  Peripheral blood mononuclear cells (PBMC) are also available fresh directly from a LeukoPak.  Apheresis-derived mononuclear cells are highly purified, containing a more pure population of mononuclear cells when compared to buffy coat.

 

Mobilized Peripheral Blood Mononuclear Cells (PBMC)

Mobilized peripheral blood mononuclear cells (PBMC) are collected from healthy human volunteer donors under IRB approved informed consent by leukapheresis using the COBE® Spectra Apheresis System MNC collection protocol.  Donors are injected with G-CSF (Neupogen® - FDA approved), which stimulates the bone marrow to produce a large number of hematopoietic and progenitor stem cells and mobilizes them into the peripheral blood stream.  This mononuclear cell-rich leukapheresis product is extracted using continuous flow centrifugal technology directly into the collection bag. Mobilized mononuclear cells are purified by a density gradient centrifugation and are available in fresh or cryopreserved format.

 

Bone Marrow Mononuclear Cells

Bone marrow mononuclear cells are collected from healthy human volunteer donors under IRB approved informed consent. Bone marrow aspirates and cells are drawn from the posterior iliac crest using a syringe containing anticoagulant. Bone marrow mononuclear cells are obtained using density gradient centrifugation techniques.  Bone marrow mononuclear cells can be further processed to isolate a variety of subpopulations and are available in a fresh or cryopreserved format.

 

Cord Blood Mononuclear Cells

Human cord blood is collected aseptically at the time of delivery from healthy pregnant women under IRB approved informed consent.  Cord blood mononuclear cells are isolated by diluting the cord blood with balanced saline and using density gradient centrifugation techniques.  Cord blood mononuclear cells are available in fresh or cryopreserved format. Cord blood mononuclear cells can be further processed to isolate a variety of subpopulations.

 

 

Serological and Infectious disease testing is performed with each donation. Customers should handle product as if potentially infectious following biosafety level 2 procedures.

 

HemaCare can provide custom products not listed in our catalog.  If different cell types/blood components, alternate formats or sizes, special donor criteria, various types of in vitro manipulation or culture are needed, we can often accommodate these special requests.  Please contact us to discuss your project and specific needs.  HemaCare has the capability to prepare and provide mononuclear cells according to your specifications:

 

Targeted Donor Selection
Our diverse donor pool enables us to provide the match you need to successfully complete your study:

  • Healthy donors
  • HLA specific (low and high resolution)
    • HLA Class I: A, B, C
    • HLA Class II: DRB, DQB, DPB, DQA
  • CMV negative/positive
  • Blood type specific
  • Diverse demographics
  • Gender and/or age specific
  • Specific lifestyle characteristics
  • Disease-state donors and patients
  • Ability to provide both repeat or unique donors

Customized Collections and Cell/Tissue Processing
Because all research projects vary, having our cells your way is central to successful research.  HemaCare can tailor collection and processing services to fit your needs.  We provide:

  • Protocol-specific collections - Your Way
  • Specific cell requests
  • Multiple cell types from same donor
  • Density separation of white blood cells
  • Positive or negative immunomagnetic cell selection methods
  • Custom primary cell cultures
  • Fresh or frozen cell formats
  • Custom cell number/volume per unit
  • Packaging and shipping to your specifications (i.e. shipping media, bu­ffers, containers)

Learn more

HemaCare can source disease state material from donors with autoimmune disorders, cancers, neurodegenerative disorders, infectious diseases, or certain rare disorders.  Some examples of disease states include:

  • Celiac Disease
  • Crohn’sDisease
  • Psoriasis
  • Rheumatoid Arthritis
  • Rheumatoid Factor (RF)
  • Systemic Lupus Erythematosis (SLE)
  • HIV, HPV, HBV & HCV positive donors

*Cord blood products are NOT available in a disease state.  Disease state bone marrow products coming soon.

 

If you don't see a product you are looking for, contact us at (877) 397-3087!

 

Currently, HemaCare sources disease state products from our partners.

 

Packaging and shipping specifications play an important role in maintaining the quality and integrity of products.  We package and ship all products to your specifications in a validated shipping box.

 

Mononuclear cells are normally packaged and shipped overnight from day of collection (fresh)/request (cryopreserved).  Unless instructed otherwise, fresh mononuclear cells are packaged with wet ice to maintain temperature of 1-6°C during shipment. Cryopreserved mononuclear cells are packaged with dry ice to maintain frozen temperature during shipment.

 

Temperature monitoring data tracking devices are available for use at your request.

 

Our healthy human volunteer donors for research products are screened with the same stringent regulations and requirements as donors who donate transfusable blood components.

 

Donors must:

  • Be at least 18 years old.
  • Weigh at least 140 lbs.
  • Be in good health and feeling well.
  • Not be pregnant.
  • Meet protocol specifications.
  • Be screened for evidence of transmissible infectious diseases (i.e. HIV, Hepatitis B, Hepatitis C).This panel of tests is repeated with each product collection.
  • Have adequate peripheral veins for apheresis.
  • Review and sign an institutional review board (IRB) approved procedure-specific consent form prior to the collection, in addition to the Donor History Questionnaire.

Maternal donors for cord blood donations must:

  • Be at least 18 years old.
  • Be screened for evidence of transmissible infectious diseases (i.e. HIV, Hepatitis B, Hepatitis C).
  • Review and sign an institutional review board (IRB) approved procedure-specific consent form prior to the collection, in addition to the Donor History Questionnaire.

HemaCare strictly adheres to all applicable Federal and State guidelines .  All donations involve complete anonymity for the donor.  In addition, HemaCare utilizes randomly generated numbers to track products and there are no identifiers that can be traced back to the donor.

 

 

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